Azza H. El-Medany
King Saudi University
Saudi Arabia.
Title: Effect of chronic exposure to diazinon on glucose homeostasis and oxidative stress in pancreas of rats and the potential role of mesna in ameliorating this effect
Biography
Biography: Azza H. El-Medany
Abstract
Residential and agricultural pesticide use is widespread in the world . Their indiscriminative use , with their ability to interact with biological systems constitute a health hazards . 40 adult male rats, were classified into three groups; control were received corn oil (10mg/kg/day) . Diazinon were received diazinon ( 10mg/kg/day ). Mesna group (15rats), were received mesna 180mg/kg once a week 15 min. before administration of diazinon s. At the end of the experiment ( 2 months ), animals were anesthetized , blood samples were taken for glucose and insulin assays & pancreas was removed and divided into 3 portions ; for histopathological ; ultrastructural & biochemical studies. A significant increase in the levels of malondialdehyde, TNF-α, myeloperoxidase activity , IL-1β , serum glucose levels in the toxicated group were observed , while a significant reduction was noticed in GSH & in serum insulin levels. After treatment with mesna a significant reduction was observed in the previously mentioned parameters except that there was a significant rise in GSH & in insulin levels. Histopathological & ultrastructural studies showed destruction in pancreatic tissues and the β cells were the most affected cells . This study shows the role of antioxidant drugs in ameliorating the toxicity that is considered as a complementary treatment of pesticide toxicity.