Fisseha Shiferie
Mekelle University,
Ethiopia
Title: Ex-vivo spasmolytic activities and In-vivo antidiarrheal of the aqueous extract of the roots of Echinopskebericho mesfi nin isolated guinea-pig and ileum rodents
Biography
Biography: Fisseha Shiferie
Abstract
Diarrhea is a common gastrointestinal disorder characterized by an increase in stool frequency and a change in stool consistency. Inspite of the availability of many drugs as antidiarrheal agents, the search for a drug with aff ordable cost and better effi cacy is essential to overcome diarrheal problems. Th e root extract of Echinopskebericho, is used by traditional practitioners for the treatment of diarrhea. However, the scientifi c basis for this usage has not been yet established. Th e purpose of the present study was to evaluate the antidiarrheal and spasmolytic activities of the aqueous extract of the roots of E. kebericho in rodents and isolated guinea-pig ileum preparations. In the castor oil induced intestinal transit test, E.kebericho produced a signifi cant (p < 0.01) dose dependent decrease in propulsion with peristaltic indexvalues of 45.05±3.3, 42.71±2.25 and 33.17±3.3%, respectively at doses of 100, 200 and 400 mg/kg compared with 63.43±7.3% for control. In the castor oil-induced diarrhea test, the mean defecation was reduced from 1.81±0.18 to 0.99 ± 0.21 compared with 2.59 ±0.81 for control. Th e extract (at doses stated above) signifi cantly decreased the volume of intestinal fl uid secretion induced by castor oil (2.31±0.1 to 2.01±0.2) in relation to 3.28±0.3 for control. When tested on a guineapig ileum, root extract of Echinopskebericho exhibited a dose dependent spasmolytic eff ect, 23.07 % being its highest inhibitory eff ect. Th e results obtained in this study give some scientifi c support to the use of Echinopskebericho as an antidiarrheal agent due to its inhibitory effects on the different diarrheal parameters used in this study.