Muhammad Idrees
University of the Punjab, Lahore
Title: IL28B TT and HCV-3a genotypes are two risk factors for the development of Hepatocellular Carcinoma
Biography
Biography: Muhammad Idrees
Abstract
Chronic HCV infection frequently leads to liver cirrhosis and is associated with an elevated risk for progression into hepatocellular carcinoma (HCC). Several host and viral factors are assumed to play a role in this process. The aim of this study was to evaluate the role of IL28B and HCV genotypes in the development HCC in chronic HCV infected Pakistani patients.
Total 161 subjects with HCC were included in this study. Liver biopsy was performed on 145 of the patients. Sixteen patients were excluded because they failed to fulfill the inclusion criteria. qPCR was performed for HBV and HCV. Samples positive for HCV RNA were genotyped using genotype specific PCR and confirmed by 5’NCR sequence analysis. RFLP and direct sequencing were performed for the genotyping of Single nucleotide Polymorphism rs12979860 of IL28B.
Chronic HCV infection was identified a major risk factor (63.44%) for the development of HCC. The time from HCV infection to appearance of cancer was 10-50 years. In the HCC patient population, broader distributions of genotypes were present with genotype 3a as the predominant genotype. Sixty-six percent of treated patients with cirrhosis had an end of treatment response, but unfortunately they relapsed quickly when the treatment was discontinued, and HCC developed during a median 3.8 years. The statistically higher T allele frequency >80% was observed in patients with HCC as compared to the C allele frequency <20% in patients who did not develop HCC (p<0. 001). Based on the results of this study, a strong association was observed between chronic HCV-3a infection, IL28B TT genotype and HCC in Pakistan